P-196: Lactation and mRNAs Expression of RFRP-3 and KiSS-1 in Hypothalamus of Rat

Authors

Abli Moghadam AA

Asadi Yousefabad SL

Jafarzadeh Shirazi MR

Niazi A

Rahmanifar F

Sabet Sarvestani F

Tamadon A

Tanideh N

Abstract:

Background: Comparison of the effect of lactation on expression of RFRP-3 mRNA in dorsomedial hypothalamic nucleus (DMH) and KiSS-1 mRNA in arcuate nucleus (ARC) of hypothalamus in lactating and nonlactating rat Materials and Methods: Fourteen rats of the Sprague- Dawley strain were randomly allotted into three groups. The non-lactating rats (n=5) were separated from their pups immediately upon parturition. The lactating rats were allowed to suckle five pups for eight days (the period of increasing of lactation). Four ovariectomized rats were selected as control group. Relative expression of RFRP-3 and KiSS-1 mRNAs (compared to the control group) respectively in DMH and ARC of hypothalamus was determined using real-time PCR. Mean of data in lactating and non-lactating groups were compared by independent sample test (SPSS 11.5; p<0.01). Results: Mean and SE of relative expression of RFRP-3 mRNA in DMH in the lactating group (62.0 ± 10.0%) was higher than that of the non-lactating rats (0.4 ± 0.1%, p=0.001). Relative expression of KiSS-1 mRNA in ARC was not different between the lactating (2.2 ± 0.3%) and the non-lactating rats (1.8 ± 0.4%, p=0.4). Conclusion: Increased of RFRP-3 mRNA expression in DMH of hypothalamus while increasing of milk production in the rats may be the inhibitory factor of GnRH secretion. Whereas no change in KiSS-1 mRNA expression in the ARC of rats may indicate lower activity of the nucleus in production of the gene in both groups postpartum.

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